Fig. 4.
![Fig. 4. Immunoprecipitation analysis of [35S]methionine-labeled lysates using anti-p21Waf1 antibody. [35S]methionine-labeled lysates of T lymphocytes stimulated for 24 hours (A) and 42 hours (B) were immunoprecipitated with anti-p21Waf1 antibody (lanes 1, 3, 5, 7, and 9) or with preimmune serum (lanes 2, 4, 6, 8, and 10). Cells were treated with the drugs indicated from time 0 (1 μmol/L MPA, 50 μmol/L guanosine, 100 μmol/L 8-aminoguanosine, 25 μg/mL 5-FU, 10 μmol/L ara-C). The mobility of protein molecular weight standards p21Waf1 and CDK6 are indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/8/10.1182_blood.v91.8.2896.2896_2896_2904/5/blod4082104a.jpeg?Expires=1722412735&Signature=X3bsKawAbMIFdGJStIMk4wDakkBSs69Ma6SJD7XL4Qpo673wS1PJ5VfU6RQFuNbZwveuWhuGlBi1mMyyebKiBrRZuu2ssaCpN7Xpug1yNqp4U3N8K60wXsv1jQsJyT-1qmVJMLpkKSZJqcbtzyF5wSLz22uPN8eWxZVFmaBkzCJ52ZFNOmanzE0Fv5Ialv80x6rtdo53poV8hZ0ugPYexRlaEpbx1W3PIMHNmH~-BslMpeQvAf~N1ipFuHtbKI9mH-3F3nnBeQtcP8T9kqSQKXGLcZ83KvG9zch0pAlOPe56vLKCrBnlMt2ZCWAp8hz8q~k3jI4gV7cy3Kr3dQ1bLA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoprecipitation analysis of [35S]methionine-labeled lysates using anti-p21Waf1 antibody. [35S]methionine-labeled lysates of T lymphocytes stimulated for 24 hours (A) and 42 hours (B) were immunoprecipitated with anti-p21Waf1 antibody (lanes 1, 3, 5, 7, and 9) or with preimmune serum (lanes 2, 4, 6, 8, and 10). Cells were treated with the drugs indicated from time 0 (1 μmol/L MPA, 50 μmol/L guanosine, 100 μmol/L 8-aminoguanosine, 25 μg/mL 5-FU, 10 μmol/L ara-C). The mobility of protein molecular weight standards p21Waf1 and CDK6 are indicated.
