Fig. 3.
![Fig. 3. Inhibition of CDK2- and CDC2-associated kinase activities by MPA. Immunoprecipitations were performed using lysates from human T lymphocytes before and after IL-2/PHA-L stimulation and anti-CDK2 (lanes 1 to 7) or anti-CDC2 (lanes 8 to 14) antibodies. Competitive peptides were added to the 48-hour samples to control for the specificity of the assay. The immune complexes were incubated in kinase buffer containing [γ-32P]ATP and histone H1 for 30 minutes, separated on a SDS-PAGE and exposed to radiograph film, as outlined in the Materials and Methods section.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/8/10.1182_blood.v91.8.2896.2896_2896_2904/5/blod4082103.jpeg?Expires=1730477032&Signature=kwKM~ielAc8duaW89khfqt7ltliyabsUr6mpv7hKIiSqLCZIHBzamk5Ux9box4HKfdHuGj459y8VOIbbIdzC8rEgG-tO3AQmxAavuQ~q4n1yA6Daihcc1U6cOxSLQQ6ymWE~gE~xfp7Lo1k8q5o4nM36rAVeYkDNTGn3xhE0-LYPj-0Swk0lb00tumGyMBIlEpVZ0f1fwD8XI7YKThGRUTJKOKiG1Ezg4t9-Q9JdtyspMSANhl4O8w7dZ4yrrMiK3-0Fmwn0EPXJFUdgDneOFbgI5S14~VYu7V7Ze-fUOJLcGmdkh5cMTYpdxNzplvlZyB2gYpZbfUlRH0tDi7U1PA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of CDK2- and CDC2-associated kinase activities by MPA. Immunoprecipitations were performed using lysates from human T lymphocytes before and after IL-2/PHA-L stimulation and anti-CDK2 (lanes 1 to 7) or anti-CDC2 (lanes 8 to 14) antibodies. Competitive peptides were added to the 48-hour samples to control for the specificity of the assay. The immune complexes were incubated in kinase buffer containing [γ-32P]ATP and histone H1 for 30 minutes, separated on a SDS-PAGE and exposed to radiograph film, as outlined in the Materials and Methods section.
