Morphologic analysis of hematopoietic precursor cells several days after infection with AdGFP. Forty-eight to 96 hours after infection of expanded precursor cells with AdGFP, fluorescence and light microscopic analysis of liquid cultures and cytospin preparations were performed. (A) Megakaryocytic cells in liquid culture demonstrated bright fluorescence 48 hours after infection (100 MOI). (B) Cytospin preparations of the same culture confirmed the presence of large multilobulated and monolobulated megakaryocytes (Wright-Giemsa staining; original magnification ×1,000). (C) Examined under UV light, these cells were brightly fluorescing (×1,000). Cytoplasm pseudopods and blebs, typical features of polyploid mature megakaryocytes, are recognizable. (D) Liquid culture of dendritic cells shown in phase-contrast microscopy before infection. (E) The same cell culture 48 hours after infection with 100 MOI of AdGFP shows numerous dendritic cells expressing GFP (×100). (F) Cytospin preparations of myelomonocytic expansion cultures 72 hours after infection (100 MOI) show morphologically normal granulocytic and monocytic precursor cells (Wright/Giemsa staining, ×600). (G) Examined with UV microscopy, mainly large monoblastic cells expressed GFP.