American Society of Hematology

Fig. 2.

Altered dimerization of BAXG67R and BAXG108V in vivo compared with wild-type BAX. (A) Immunoprecipitation of BCL-2 from 0.25% NP-40 lysates of35S methionine/cysteine-labeled FL5.12-BCL-2 cells (Hygro) or HA-BAX, HA-BAXG67R ,or HA-BAXG108V stably transfected clones using antihuman BCL-2 MoAb 6C8 and SDS-PAGE.3 (B) Immunoprecipitation and sodium dodecyl sulfate-PAGE analysis using anti-HA MoAb 12CA5 from 0.25% NP-40 lysates of 35S methionine/cysteine-labeled FL5.12-BCL-2 cells (Hygro) or HA-BAX, HA-BAXG67R, or HA-BAXG108V stably transfected clones.

Altered dimerization of BAX^G67R and BAX^G108V in vivo compared with wild-type BAX. (A) Immunoprecipitation of BCL-2 from 0.25% NP-40 lysates of³⁵S methionine/cysteine-labeled FL5.12-BCL-2 cells (Hygro) or HA-BAX, HA-BAX^{G67R ,}or HA-BAX^G108V stably transfected clones using antihuman BCL-2 MoAb 6C8 and SDS-PAGE.3 (B) Immunoprecipitation and sodium dodecyl sulfate-PAGE analysis using anti-HA MoAb 12CA5 from 0.25% NP-40 lysates of ³⁵S methionine/cysteine-labeled FL5.12-BCL-2 cells (Hygro) or HA-BAX, HA-BAX^G67R, or HA-BAX^G108V stably transfected clones.

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