American Society of Hematology

Fig. 5.

$Fig. 5. Generation of total progenitors after infection and liquid culture of sorted 200 CD34+ cells. One-milliliter cultures were initiated with 1.0 × 104 sorted CD34+ cells in the StemPro-34 SFM Complete Medium containing IL-6 and SCF in the presence or absence of EGF. These cells were incubated with the Ax1w or Ax/hEGFR virus, and control cells were cultured without adenovirus vectors. After 5 days of incubation, 1/50 of the cells of each fraction were subjected to methylcellulose clonal culture with SCF, IL-6, IL-3, and Epo in triplicate. The data presented are from a single patient, and the mean ± SD of results from three dishes is shown.$

Generation of total progenitors after infection and liquid culture of sorted 200 CD34⁺ cells. One-milliliter cultures were initiated with 1.0 × 10⁴ sorted CD34⁺ cells in the StemPro-34 SFM Complete Medium containing IL-6 and SCF in the presence or absence of EGF. These cells were incubated with the Ax1w or Ax/hEGFR virus, and control cells were cultured without adenovirus vectors. After 5 days of incubation, 1/50 of the cells of each fraction were subjected to methylcellulose clonal culture with SCF, IL-6, IL-3, and Epo in triplicate. The data presented are from a single patient, and the mean ± SD of results from three dishes is shown.

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