Flow cytometric analysis of BM from a healthy donor compared with an FAB-classified AML-M3 patient. Double labeling was performed with AAA4-FITC (NAMLAA specific) and CD15-PE. The scatter morphology of the BM sample and the control stainings with IgG-FITC and IgG-PE are given in the two upper plots. Double-labeling of BM cells with the antibody AAA4-FITC and the granulocyte marker CD15-PE shows that in the normal situation (lower left) the (immature) granulocytes are CD15 and NAMLAA double positive, whereas the AML cells are only positive for NAMLAA.