The FAC/cdc2 interaction correlates with the functional activity of FAC. (A) HSC536N (mock) cells and HSC536N (+FAC) cells were harvested following MMC treatment, stained with propidium iodide, and analyzed by FACS, as described in Materials and Methods. (B) Whole cell extracts from HSC536N (mock) cells (lanes 2 and 4) or HSC536N (+FAC) cells (lanes 1 and 3) were immunoprecipitated with affinity purified anti-FAC antisera prepared from two different New Zealand white rabbits. Immunoprecipitated proteins were electrophoresed by SDS-PAGE, blotted to nitrocellulose, and probed with anti-cdc2 antiserum or anti-FAC antiserum. Molecular weight markers are in kilodaltons.

(C) Whole cell extracts from HSC536N (mock) cells (lane 1) or HSC536N (+FAC) cells (lane 2) were immunoprecipitated with anti-cdc2 monoclonal antibody. Immunoprecipitated proteins were electrophoresed by SDS-PAGE, blotted to nitrocellulose, and probed with affinity purified anti-FAC antisera. Molecular weight markers in kilodaltons.