Fig. 8.
![Fig. 8. Effect of thrombin on CD45 and Lck reconstituted deficient clones. (A) Thrombin-induced [Ca2+]i responses. JE6.1 (□), J45.01 (⊞), J45.01/CD45 (⧫), JCaM1 (▴), or JCaM1/Lck (◊) were stimulated with 100 nmol/L thrombin and fluorescence analyzed as a function of time. (B and C) Thrombin-mediated phosphorylation of p38MAPK. Cells were stimulated with 100 nmol/L thrombin for the times indicated. Proteins from cell lysates were subjected to SDS-PAGE as described in the legend to Fig 4 and were transferred to Immobilon membranes for Western blotting using phospho-specific p38 MAPK antibody. Equal amounts of p38 MAPK were detected in each condition (not shown).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/11/10.1182_blood.v91.11.4232/4/blod41132008w.jpeg?Expires=1722546871&Signature=lomfWWUleBjDPT0uh37HpZk8Tu7oCyRDJcYSmZ4oFynrdjFResC67mjikUXxCm~chyd0SBZlbcxPmaa8uoRyUHvOQdF642i4Cja8ttMfKku8P-8Lmj-lVemPJIuSkw9k~YQowEcTUNuNxYL5~NulQw~07AOQgUHw-QtTvPlw44lAY7lTLIgAXyt7kJhZz6SRjiuClkysLiI8F-Tq9yoPBvaXDAHDnhp~YdV5BLh-L5Ljgp3DkSARQnSOTR~wbY7E2hbgZaRBBdwf5dzFy2pS2n3WLDmoiuWCSxnZPgN64hNJv1VxQY0CZirJuSZ6lshZR68e4pWzg8Mj3vWSRr829w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of thrombin on CD45 and Lck reconstituted deficient clones. (A) Thrombin-induced [Ca2+]i responses. JE6.1 (□), J45.01 (⊞), J45.01/CD45 (⧫), JCaM1 (▴), or JCaM1/Lck (◊) were stimulated with 100 nmol/L thrombin and fluorescence analyzed as a function of time. (B and C) Thrombin-mediated phosphorylation of p38MAPK. Cells were stimulated with 100 nmol/L thrombin for the times indicated. Proteins from cell lysates were subjected to SDS-PAGE as described in the legend to Fig 4 and were transferred to Immobilon membranes for Western blotting using phospho-specific p38 MAPK antibody. Equal amounts of p38 MAPK were detected in each condition (not shown).
