Partial CGH karyotypes of cases with high-level DNA amplifications. Images were acquired and evaluated using the CYTOVISION software package (Applied Imaging). Numbers at the bottom indicate the respective chromosomes. Hybridization with the tumor DNA is shown as gray level images. The band-like hybridization signals (arrows) indicate highly amplified chromosomal sequences. On the right side of the ideograms, the average ratios of FITC/rhodamine fluorescence are plotted (“ratio profiles”). The central line indicates a ratio value of 1.0; lines to the right indicate ratio values of 1.25 and 1.5, respectively; and lines to the left indicate ratio values of 0.75 and 0.5. n = number of chromosomes analyzed for calculating the respective average ratio profile.