Fig. 7.
Expression of PGI2-R and PF4 mRNA in normal human megakaryocytes as evaluated by semiquantitative RT-PCR. More than 200 megakaryocytic colonies were plucked and pooled on the indicated days of culture, total RNA was extracted, and RT-PCR was performed. The amount of transcripts for PGI2-R or PF4 was analyzed and compared with that of G3PDH. M, size marker (φX174/HaeIII digest). This figure shows the representative results from experiments repeated three times.
