Fig. 6.
![Fig. 6. Effect of PMA and TPO on PGI2-R expression. HEL () and NS-Meg (▪) cells were preincubated for 48 hours with or without (control) either PMA or TPO. PGI2-R expression was evaluated by specific [3H]iloprost binding to the cells. Values (mean ± SE, n = 4) are expressed as a ratio of scintillation count of the control cells (nontreated HEL, 1.3 × 105 ± 5.3 × 103 dpm/1 × 106 cells, nontreated NS-Meg, 4.2 × 104 ± 3.9 × 103). Preincubation with PMA or TPO increased the binding capacity of the ligand up to sixfold and threefold in HEL cells and fivefold and twofold in NS-Meg cells, respectively, when compared with control values. The experiment is representative of three performed.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/3/10.1182_blood.v90.3.1039/4/bl_0006f6.jpeg?Expires=1722426039&Signature=yqe8DI4oxYCxNkNjHDagYF1cNb6ujOcbEPcTyThNAeJIkV3xq2s4H3~8zmg72pQhVeA38KqYphRGsrRu9UkoIPAQv4x1gzyy0l4HytobJLZvDKBTiiveq5ENcQ54w4IPV8ymtejpRzgpcFOl26jEG~u356LmwsTppDkS8jaRKCBiWAG39X-InKutnSc-yjWRm-rKeqTYo5CN7M9tDai0IqfHBecxEBc8NCZvuUW6n~6af7L-GjVjCzZbDI~Di5drma0RP5StS6hCPvUCh0bp2wpzu-b-0O568aFwp1EF-CYAlc-gpZwrlyKuZwM8e3CiMCA5EDICmEmSGlCOitmWqQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of PMA and TPO on PGI2-R expression. HEL () and NS-Meg (▪) cells were preincubated for 48 hours with or without (control) either PMA or TPO. PGI2-R expression was evaluated by specific [3H]iloprost binding to the cells. Values (mean ± SE, n = 4) are expressed as a ratio of scintillation count of the control cells (nontreated HEL, 1.3 × 105 ± 5.3 × 103 dpm/1 × 106 cells, nontreated NS-Meg, 4.2 × 104 ± 3.9 × 103). Preincubation with PMA or TPO increased the binding capacity of the ligand up to sixfold and threefold in HEL cells and fivefold and twofold in NS-Meg cells, respectively, when compared with control values. The experiment is representative of three performed.
