Fig. 4.
Specific recognition of HLA-Dw26–positive BM early progenitor cells by T-cell clone 6.2. (A) HLA-Dw26–positive or –negative BM cells from healthy individuals were used to stimulate clone 6.2 (left panel) and the control HLA-Dw26–specific alloreactive T-cell clone (right panel) in 88-hour standard proliferation experiments. (B) MoAb 7.3.19.1 is added in the assay. (C) Different subsets of BM cells were obtained by FACS sorting after labeling with appropriate antibodies, irradiated, and used in proliferation experiments as stimulator cells at 5 × 104 cells/well.
