NO-induced activation of FLICE in lymphoid cell lines. (A) Time-dependent effect of NO in APO-S and BL60 cells. (B) Inhibition of FLICE activation upon treatment with protein synthesis inhibitor cycloheximide (CHX) in APO-S but not in BL60 cells. Cells were treated with 0.2 mmol/L GTN for the time period indicated and/or with CHX at different concentrations. Western blot analysis with anti-FLICE MoAb was performed. The full-length FLICE and the p18 active cleavage product are presented.