Fig. 3.
NO-induced apoptosis in freshly isolated human leukemic cells requires caspase activity and involves the CD95 pathway. PBMC from a patient with T-ALL isolated by Lymphoprep separation were plated at a density of 5 × 105 cells/mL and incubated for 24 hours with anti-CD95 (10 μg/mL), or broad-spectrum caspase-specific inhibitor zVAD-fmk (zVAD; 20 μmol/L) or with F(ab′)2 anti-CD95 blocking antibody (1 μg/mL) and/or GTN at the concentrations indicated. Percentage of apoptotic cells was measured by the DNA-release assay.
