Induction of CD95L (A) and TRAIL/APO-2L (B) mRNA expression by GTN in APO-S Jurkat cells. Cells were treated for different periods of time with 0.2 mmol/L GTN, and total RNA was isolated. After semi-quantitative RT-PCR with specific primers for CD95L or TRAIL/APO-2L, PCR products were run on a 1.5% agarose gel. APO-S cells stimulated with PMA (20 ng/mL; Sigma) and ionomycin (1 μmol/L; Calbiochem) for 6 hours were used as positive control. Both pictures were scanned using the Adobe Photoshop and the Scan Analysis programs. The ratio between the densities of the specific band to the β-actin band is shown in the histograms on the lower panel (indicated in arbitrary units). (A) The amplified products for CD95L (447 bp) and β-actin (661 bp) are labeled. The gel was blotted and hybridized with CD95L and β-actin–specific probes. (B) The amplified products specific for TRAIL (422 bp) and β-actin (661 bp) are presented.