Plots of the binding of β₂GPI to liposome membranes containing CL as a function of the concentration of CL. One micromolar of intact (▪) or nicked (•) β₂GPI was incubated with CL/PC (1:1)-LUV at 25°C in 10 mmol/L Tris-HCl buffer containing 10 mmol/L NaCl. The amounts of proteins bound to liposomes were measured as described by Hagihara et al.11