Fig. 4.
![Fig. 4. (A) HiTrap Heparin column chromatography patterns of plasmin-treated β2GPI. A total of 660 μg of β2GPI was incubated with 19 μg of plasmin at 37°C, where molar ratio of plasmin to substrate was 1:56. A total of 60 μg of the digest of β2GPI was subjected to HiTrap Heparin column chromatography, as described in Materials and Methods. (B) Time course of the increase of nicked β2GPI (peak 1 in [A]). Horizontal axis and vertical axis correspond to the reaction time (hours) and area of peaks of nicked β2GPI. The experiment was performed in the presence (•) or absence of α2PI (▪).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/11/10.1182_blood.v91.11.4173/4/blod41135004bx.jpeg?Expires=1722540483&Signature=LDkCMiZrt9qhF89b5I1PaY6rQX68YI6RyyFTUblGJb--ZANnMcoe7dvAMN3SM~vwPS0JaKzchWNxTmikXsdJPxtFRCLrxuxxvaUpE5RFfNuOjwevgRHR67bmflzMhkrYLY-1CmcqZNHAL7vMHtbiYlXNkukLjt5UzV4VnB-j6LO0El3TIzf0ybTceB-~XYm04iaIGX0uBKGD0~NM9nWIDHXPZqTrDLZDLrPFSRQrw~vrhkrrjwmCqsvL3IXvUoXyT2I5lx0C0RnEHU0LZTsLDW68Zqmblqn-jtt7quNzYyKCOjwLJEOT8vY6VZwOmjzJphcrL4mnFal--cZoKJT2mw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
(A) HiTrap Heparin column chromatography patterns of plasmin-treated β2GPI. A total of 660 μg of β2GPI was incubated with 19 μg of plasmin at 37°C, where molar ratio of plasmin to substrate was 1:56. A total of 60 μg of the digest of β2GPI was subjected to HiTrap Heparin column chromatography, as described in Materials and Methods. (B) Time course of the increase of nicked β2GPI (peak 1 in [A]). Horizontal axis and vertical axis correspond to the reaction time (hours) and area of peaks of nicked β2GPI. The experiment was performed in the presence (•) or absence of α2PI (▪).
