Fig. 1.
![Fig. 1. Size exclusion HPLC analysis of cytosolic (A) and nuclear (B) extracts prepared from fresh blasts from the PB of an APL patient expressing the bcr3-PML/RARα isoform. Extracts (200 μL) were incubated with 10 nmol/L [3H]-t-RA in the absence (•) or in the presence (○) of 200-fold mol/L excess of unlabeled t-RA for 18 hours at 4°C. The samples were then fractionated over a Superose 6 HR 10/30 size exclusion column as described in Materials and Methods.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/3/10.1182_blood.v90.3.1175/4/bl_0029f2b.jpeg?Expires=1722748579&Signature=nkm8erk4lLMTGOyCpNsCNwBbpnrJr3koIXChObj8ehK-NibLWXEICLJgO2rU8GjSFEVSWmmpghW76P9QOOlKLLIYvR64Bk9y29uMHrSehmOfJhLSSQcHr-Dx2eQyDRe87ESjPC51gKGGyEzntZoJUqJ2w7Sqn3K81xsqH5LVJPM7Sk~~2Z~ogbBt3xaE-zo4-0JLa2togNF7ZaK1qrKj7x2X4uRjTL~gSxIiGKbE~XFzozDDKnkLM7Hi8NRGK7HWIGAL2sCosQYs65a7YzVSSurhfBjvmcD5jvqqOuNhwBXG1mLue8Aw9JJ7CFGIUNxZ80MCAIvQQmxyrDVh8n8-Ow__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Size exclusion HPLC analysis of cytosolic (A) and nuclear (B) extracts prepared from fresh blasts from the PB of an APL patient expressing the bcr3-PML/RARα isoform. Extracts (200 μL) were incubated with 10 nmol/L [3H]-t-RA in the absence (•) or in the presence (○) of 200-fold mol/L excess of unlabeled t-RA for 18 hours at 4°C. The samples were then fractionated over a Superose 6 HR 10/30 size exclusion column as described in Materials and Methods.
