Cumulative nonadherent cell production by LTBMCs treated with TN-C glycoprotein in Whitlock-Witte's culture condition from BM cells of TN-C–deficient mutant mice (a) and control mice (b) obtained at 7 weeks of age. Results represented as cumulative mean ± SD of nonadherent cells produced weekly by at least four cultures per group per week. TN-C was added to LTBMCs at final concentration of 4 ng/mL (▵), 20 ng/mL (□), 100 ng/mL (⧫), and without TN-C (•). Results were significantly different between TN-C–treated and TN-C–nontreated cultures (P < .01) in both culture systems. Two other identical experiments gave comparable results.