Fig. 1.
![Fig. 1. Amplified wt1-specific mRNA and β-actin–specific mRNA in AML blast cells (length of PCR product: 857 bp [wt1] and 652 bp [β-actin]). Lane 1, marker (100-bp ladder, Hind/EcoRI-digested λ DNA); lane 2, negative control; lane 3, positive control (cell line HEL 92.1.1); lanes 4 and 5, wt1-negative AML; lanes 6 and 7, wt1 low AMLs; lanes 8 and 9, wt1 intermediate AML; lanes 10 and 11, wt1 high AMLs. Amount of wt1-specific mRNA was determined by quantitative PCR: lanes 4 and 5, <0.0001 fg/μg total RNA; lane 6, 0.023 fg/μg; lane 7, 0.031 fg/μg; lane 8, 0.186 fg/μg; lane 9, 0.367 fg/μg; lane 10, 19.037 fg/μg; lane 11, 9.484 fg/μg.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/3/10.1182_blood.v90.3.1217/4/bl_0030f1.jpeg?Expires=1722779458&Signature=LKC4j-MTioX7oCykKMC339278dj~Wf0jZCfew4DQYFhElOmoR82z4s3ZtHrRCxNcmAQZ3YdZzvf4Qzd~Gu-0EtoDc0pz9ny8hfegOwQ-8CjQVuFKgJRTEYxFtftqTBZY2qpDGQDYa6pnFPcZpC~rWuuctspYSa5ijRXTokAUI0RLFrTMu3y7peRS94LoPS0HedfEecDSxj0i-CxLztdVcBUN2UsDRulX-WQcxUfrfDP8Jm633W5J4djqK2Qi1wi6UOpCDIR8liZD7gPCxwdtCK5oV6TBKE9JTHcw~8jMIz3kbOSCIiFXBQ0lwHWls6MjLaP~q0kKWA79govVavLUcg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Amplified wt1-specific mRNA and β-actin–specific mRNA in AML blast cells (length of PCR product: 857 bp [wt1] and 652 bp [β-actin]). Lane 1, marker (100-bp ladder, Hind/EcoRI-digested λ DNA); lane 2, negative control; lane 3, positive control (cell line HEL 92.1.1); lanes 4 and 5, wt1-negative AML; lanes 6 and 7, wt1 low AMLs; lanes 8 and 9, wt1 intermediate AML; lanes 10 and 11, wt1 high AMLs. Amount of wt1-specific mRNA was determined by quantitative PCR: lanes 4 and 5, <0.0001 fg/μg total RNA; lane 6, 0.023 fg/μg; lane 7, 0.031 fg/μg; lane 8, 0.186 fg/μg; lane 9, 0.367 fg/μg; lane 10, 19.037 fg/μg; lane 11, 9.484 fg/μg.
