Immunofluorescence analysis of Namalwa transfectants expressing CD44H, CD44ex7-14, CD44ex10-14, CD44ex10, or vector only. Two independently derived representative Namalwa transfectants expressing each of the CD44 variants used (CD44H_{I and II}, CD44ex7-14_{I and II}, CD44ex10-14_{I and II}, CD44ex10_{I and II}) or vector alone were phenotyped with MoAbs directed against a conserved framework CD44 epitope (solid line), an 8aa sequence encoded by CD44ex10/v6 (dotted line), or an isotope-matched negative control antibody (shaded black). CD44H transfectants reacted with the CD44 framework antibody but not the CD44ex10 (v6) antibody whereas CD44ex10, CD44ex10-14, and CD44ex7-14 transfectants reacted with both antibodies. Vector-only transfectants reacted with neither CD44 antibody.