Vβ-Cβ VDJ junction CDR3-size distribution profiles in skin tumor (A) and PBL (B) from patient Cou. cDNA made from total RNA extracted from the tumor biopsy sample or the PBL was subjected to PCR amplification with Vβ-specific primers and a fluorescent Cβ primer. The labeled PCR products were subjected to electrophoresis and analysis on an automated DNA sequencer. The fluorescence profiles (x-axis, Vβ-Cβ size in nucleotides; y-axis, fluorescence intensity) of the 24 Vβ subfamilies are shown.