Analysis of the PCR products obtained from genomic DNA in the skin tumor. Two microliters of the DNA sample were amplified with a 30-cycle PCR, using primers specific for Vβ genes in combination with Jβ primers. The PCR products were analyzed by electrophoresis through a 2% agarose gel. Lane E, size markers (100 bp); lane 1, Vβ7/Jβ2.3; lane 2, Vβ13/Jβ2.5; lane 3, Vβ22/Jβ2.5; lane 4, Vβ5/Jβ1.1; lane 5, Vβ5/Jβ1.2; lane 6, Vβ5/Jβ2.1; lane 7, Vβ5/Jβ2.7; lane 8, Vβ8/Jβ1.1; lane 9, Vβ8/Jβ1.2; lane 10, Vβ8/Jβ2.1; lane 11, Vβ8/Jβ2.7.