Fig. 2.
Differential regulation of IL-12(p40) and IL-10 in individual donors and monocytes. IL-12(p40) (▾) and IL-10 (▵) were induced in whole-blood cultures with 250 ng/mL LPS and in the presence of various concentrations DEX. The results of three individual donors are shown (A, B, and C). Monocytes stained with PE-conjugated anti-CD14 antibodies were sorted with a flow cytometer to a purity of more than 98% and cultured as described in Materials and Methods (D). The results obtained with two individual donors are shown. Cells were stimulated with LPS in the absence (▪) or presence (□) of 10−6 mol/L DEX or in the presence of 10−6mol/L DEX and 10−6 mol/L RU486 (▨). The results are expressed as a percentage of the initial response with LPS.
