American Society of Hematology

Fig. 2.

$Fig. 2. Inhibition of activity of recombinant human factor V des B (rHFV des B) by IgG fractions. The individual IgG preparations were incubated with ≈3 nmol/L rHFV des B in conditioned media at the concentrations shown for 15 minutes at 37°C. The sample was then diluted 1:10 into 50 mmol/L Tris-HCl, pH 7.9, 175 mmol/L NaCl, 5 mg/mL BSA, and residual activity was determined by the chromogenic prothrombinase assay as described in Materials and Methods. The IgG fractions were H1 (▪); H3 (□); RS (⧫); RB (◊); WH (•); AR (○); EM (▴); RH (▵); H4 (); H5 (); MJ (); and BH ().$

Inhibition of activity of recombinant human factor V des B (rHFV des B) by IgG fractions. The individual IgG preparations were incubated with ≈3 nmol/L rHFV des B in conditioned media at the concentrations shown for 15 minutes at 37°C. The sample was then diluted 1:10 into 50 mmol/L Tris-HCl, pH 7.9, 175 mmol/L NaCl, 5 mg/mL BSA, and residual activity was determined by the chromogenic prothrombinase assay as described in Materials and Methods. The IgG fractions were H1 (▪); H3 (□); RS (⧫); RB (◊); WH (•); AR (○); EM (▴); RH (▵); H4 (); H5 (); MJ (); and BH ().

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