⁵⁹Fe incorporation in lymphobastoid cells and ferritin. (A) Cells were incubated for 18 hours in RPMI supplemented with 10 μmol/L ⁵⁹FeNTA and 200 μmol/L ascorbic acid, washed, and the radioactivity in the soluble homogenates counted. The values were normalized on the data of the control cells. Means and standard deviation (SD) of three independent experiments are shown. (B) The soluble homogenates from the same number of cells were subjected to electrophoresis under nondenaturing conditions and exposed to autoradiography. Ferritin mobility is indicated by the arrow. (C) Means and SD of densitometometric quantitation of three independent experiments, as in (B), are shown. Values normalized on the control cells.