Fig. 3.
![Fig. 3. Inhibitory effect of PAR3 IgG on α-thrombin–induced calcium mobilization in mouse platelets. Mouse platelets were loaded with fura-2 and cytosolic free Ca2+ concentration [Ca2+]i (nmol/L) was measured fluorometrically.14 Extracellular calcium was chelated to prevent platelet aggregation. Platelets were preincubated with the indicated IgG preparations for 5 minutes at 37°C and then transferred to a fluorometer at room temperature. α-Thrombin (0.5 nmol/L) or U46619 (3 μmol/L) was added at approximately 100 seconds. The data shown are representative of three experiments with thrombin and two experiments with U46619.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/11/10.1182_blood.v91.11.4152/4/blod41122003x.jpeg?Expires=1723342718&Signature=mcPumakdkDWfHbPJmw~zq6ZWhEbAwLYB3m1SPyLf2rgvaS3usBm5g-rNfMr~nO7cA1Js1eCnPJyVYjFoVOTFHynkiht8slw5Bj54vFHz7SO0y3zv8RPKdOBFdMjPM17BDe86YfMRW~4wO4yBl3uBsnkSxevO-ttayvjZvin~7VsQk8fky2SpCjtbSmsGFsNvvGkD4XinScyYYulgCFqE0u3-E9KYwI2be9ZI~ThcyoeMhUUr1SaP87wnPIF7IEer8fcOE~E-3O-V8v4fGwmQHq2Hcfh1Lkp~LIF-5hd2WaDa5Dd7eenvB~7VK58Iq13l92ifZhr2B8fnuhUyiipjFA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibitory effect of PAR3 IgG on α-thrombin–induced calcium mobilization in mouse platelets. Mouse platelets were loaded with fura-2 and cytosolic free Ca2+ concentration [Ca2+]i (nmol/L) was measured fluorometrically.14 Extracellular calcium was chelated to prevent platelet aggregation. Platelets were preincubated with the indicated IgG preparations for 5 minutes at 37°C and then transferred to a fluorometer at room temperature. α-Thrombin (0.5 nmol/L) or U46619 (3 μmol/L) was added at approximately 100 seconds. The data shown are representative of three experiments with thrombin and two experiments with U46619.
