Viability of FL5.12 clones expressing Flag-Bcl-2 or Flag-Bcl-XL after treatment with γ-irradiation. (A) Cells were seeded at 1 × 105 cells/mL in triplicate wells in media containing IL-3 following increasing doses of γ-irradiation. Cells were lysed at 72 hours and analyzed by flow cytometry as described in Fig 2. No statistical significance was found between Flag-Bcl-2 and Flag-Bcl-XL clones at each dose of γ-irradiation (P < .05, Student's t-test). (B) Cell viability was determined as above at each time point following γ-irradiation. Cells were seeded at 1 × 105 cells/mL in triplicate wells and analyzed by flow cytometry as described in Fig 2. No statistical significance was found between Flag-Bcl-2 and Flag-Bcl-XL clones at each time point (P < .05, Student's t-test). Graphs shown in A and B are the mean ± SD of triplicate cultures and representative of at least two separate experiments.
