SSCP analysis of plasminogen gene exon 7 in patient 1, her healthy parents, and her healthy sister. Radiolabeled single-stranded PCR products of plasminogen exon 7 were electrophoresed in a nondenaturing 5% polyacrylamide gel as described in Materials and Methods. Lane 1, healthy control (C); lane 2, patient 1 with ligneous conjunctivitis (II-1); lane 3, healthy mother of patient 1 (I-1); lane 4, healthy father of patient 1 (I-2); lane 5, healthy sister of patient 1 (II-2). dsDNA, double-stranded DNA.