Fig. 3.
Pulse-chase analysis of tie receptor expression by HUVECs and CHO cells. CHO, clone 37 cells (A) or HUVECs (B) were preincubated in methionine-, cysteine-free media for 30 minutes to deplete intracellular amino acid pools. 35S-Translabel was added for a 30-minute pulse followed by incubation in complete growth media for the indicated chase period. Cell lysates were prepared as described in Materials and Methods and immunoprecipitated with anti-tie MoAb 5D2. Immunoprecipitates were analyzed by SDS-PAGE and autoradiography. The chase period is indicated across the top of each lane. Molecular weight markers as indicated.
