Histochemistry, enzyme cytochemistry, and immunohistochemistry of cells in 4-week cultures of BALB/c mBMMC that possess mono- (a through g) and multi-lobular (h through n) nuclei. Cytocentrifuge preparations of cells were evaluated for toluidine blue reactive granules (a, b, h, i) that contained chloroacetate esterase enzymatic activity (d, k), mMCP-5 protein (e, l), mMC-CPA protein (f, m), or mMCP-6 protein (g, n). Cells also were evaluated immunohistochemically for their surface expression of c-kit (c, j).