Fig. 5.
![Fig. 5. PCR analysis of CD23 expression in SMS-SB and other B-lymphoid cell lines. Total cellular RNA was prepared from a range of B-lymphoid and keratinocyte cell lines, and from SMS-SB cultured either in PFHMII or in serum-containing medium. Total cellular RNA was used as template for RT-PCR using the primers indicated and the products were separated by agarose gel electrophoresis and probed with a [32P]-labeled insert from the plasmid pCDL SRα296CD23. Hybridization was visualized by autoradiography.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/1/10.1182_blood.v90.1.234/5/bl_0013f5.jpeg?Expires=1722806297&Signature=4EXdXayPnbopFVaOHiUxVJ18zaCJyOE66TqLdxombGa4ZDLz3EPVbnBQ8brfv0~GKHbu1z5RXLo102umP2l2YbHsNWiu0gfDPf3uoY8yUXHVCAdMf9YWzxkfVgt0odvALuGVDplQrSqFtWmBZyW0PsxJCKu6IrRivxuqtHz06E~83NNwP9Txp4YYtetkIW6yuCej~XGd1nKNaFsBPqdyKShPZQSx7yqIXn8AbhPtyjz8u~19NVnuL4FcH3mfFpzQr8mJH1MwEdxMi6DLhcs356HrX29G~XZPH95rf6bXlbG8rEcp7nUvLIyNOhsXUCKa2Wv-OYowucez~aFD4MRIzw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PCR analysis of CD23 expression in SMS-SB and other B-lymphoid cell lines. Total cellular RNA was prepared from a range of B-lymphoid and keratinocyte cell lines, and from SMS-SB cultured either in PFHMII or in serum-containing medium. Total cellular RNA was used as template for RT-PCR using the primers indicated and the products were separated by agarose gel electrophoresis and probed with a [32P]-labeled insert from the plasmid pCDL SRα296CD23. Hybridization was visualized by autoradiography.
