Amount of mRNA Coimbra and mRNA Mondego. N, normal cDNA fragment; C, Coimbra cDNA fragment; M, Mondego cDNA fragment. Two different RT-PCR amplification products were used. They were digested with NlaIII (+, 403- + 270-nt bands; −, 673 nt) and Bsu36I (+, 524- + 372-nt; −, 896 nt), respectively, and electrophoresed. The fragments were stained with ethidium bromide and scanned. Parentheses contain the peak surface areas (arbitrary units).