American Society of Hematology

Fig. 4.

$Fig. 4. Mono-Q-Sepharose profiles of cytosolic (A) and membrane (B) fractions from normal (•) and PV (○) ECFC. Day-6 ECFC were collected and lysed with a Dounce homogenizer in buffer A without Triton X-100. Cytosolic and membrane fractions were obtained by ultracentrifugation at 100,000g for 60 minutes as described in Materials and Methods. Approximately 1 mg samples were loaded on the mono-Q column. PTP activity was measured with 32P-labeled ENDYINAL and the data are expressed as cpm obtained from 2 μL of each 0.5-mL fraction eluted from the column. A representative figure from three experiments with similar results is shown. Each experiment was performed with blood samples from different normal volunteers and PV patients.$

Mono-Q-Sepharose profiles of cytosolic (A) and membrane (B) fractions from normal (•) and PV (○) ECFC. Day-6 ECFC were collected and lysed with a Dounce homogenizer in buffer A without Triton X-100. Cytosolic and membrane fractions were obtained by ultracentrifugation at 100,000g for 60 minutes as described in Materials and Methods. Approximately 1 mg samples were loaded on the mono-Q column. PTP activity was measured with ³²P-labeled ENDYINAL and the data are expressed as cpm obtained from 2 μL of each 0.5-mL fraction eluted from the column. A representative figure from three experiments with similar results is shown. Each experiment was performed with blood samples from different normal volunteers and PV patients.

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