American Society of Hematology

Fig. 2.

$Fig. 2. Mono-Q-Sepharose separation profiles of whole extracts from normal (A) and PV (B) day-6 ECFC. Approximately 2 mg of whole cell extracts were loaded onto the column. PTP activity was measured with 32P-labeled ENDYINASL as a substrate and data are expressed as cpm obtained from 2 μL of each 0.5-mL fraction eluted from the column. Solid lines represent PTP activity; dashed lines denote NaCI gradients. (Insets) Western blot analyses of corresponding fractions with anti–SHP-1 and anti-CD45 antibodies. A representative figure from four experiments with similar results is shown. Each experiment was performed with blood samples from different normal volunteers and PV patients.$

Mono-Q-Sepharose separation profiles of whole extracts from normal (A) and PV (B) day-6 ECFC. Approximately 2 mg of whole cell extracts were loaded onto the column. PTP activity was measured with ³²P-labeled ENDYINASL as a substrate and data are expressed as cpm obtained from 2 μL of each 0.5-mL fraction eluted from the column. Solid lines represent PTP activity; dashed lines denote NaCI gradients. (Insets) Western blot analyses of corresponding fractions with anti–SHP-1 and anti-CD45 antibodies. A representative figure from four experiments with similar results is shown. Each experiment was performed with blood samples from different normal volunteers and PV patients.

This Feature Is Available To Subscribers Only