ELISAs for GM-CSF and IFNγ production of tumor-derived LGL cell lines. (A) GM-CSF ELISA showing production of soluble GM-CSF by all four cell lines (f197, 867± 75 pg/mL; m388, 620± 56 pg/mL; F8, 127 ± 9 pg/mL; and SC, 141 ± 9 pg/mL). The production of GM-CSF between IL-2–dependent cell lines (f197 and m388) is significantly different from that of the IL-2–independent cell lines (F8 and SC; **P < .01). (B) IFNγ ELISA showing production of soluble IFNγ by all four cell lines (f197, 288 ± 18 U/mL; m388, 212 ± 15 U/mL; F8, 119 ± 12 U/mL; and SC, 108 ± 11 U/mL). Similarly, the production of IFNγ between IL-2–dependent cell lines and IL-2–independent cell lines is significantly different (**P < .01).