Fig. 5.
![Fig. 5. Effect of UV irradiation on the incorporation of dFdC or F-ara-A. Exponentially growing CCRF-CEM cells were treated without (○) or with 10 J/m2 UV light (•). Then cells were immediately labeled with 0.8 nmol/L [3H]dFdC (1.5 × 10−2 μCi/mL) (A) and 120 nmol/L [3H]F-ara-A (1.5 μCi/mL) (B) in the presence of 1.0 μmol/L BrdUrd and 10 nmol/L FdUrd for 13 hours. DNA isolation and CsCl ultracentrifugation were performed as described in Materials and Methods. Fractions were collected for the measurement of radioactivity.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/1/10.1182_blood.v90.1.270/5/bl_0051f5.jpeg?Expires=1724298931&Signature=FszMGe4QGSt0drZi303fJL3xIk1tvF9iBL3IJJzhIR0jfpobQcV--jQjg-Z1HHb27OZPtNjU91Ds6zdZ6gWqEAtw6~61AywsZ3sSqMcYtpqVpnENWabmPdSk1pWNMMD1hM4EcPJVbOB~RgtClV0UMJTmhnEtUQr7NRGiu2IgYSs0jcUYs8nGoRam9Apd8dZXoJ9Oj-4KJqHB5Hw9fyMEj9tjrN8FZLIgtW~ZarMxBW-5iWoGfEmwp2Ivj2AhFpRLxsvOgSIWkBqmUlBmulnMsalGUmxawY5Kc0hk0EGUw8KDKBZYEIARdlp-X6CUt6t71sJG~5Plx1x2~X-Ve1ulCA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of UV irradiation on the incorporation of dFdC or F-ara-A. Exponentially growing CCRF-CEM cells were treated without (○) or with 10 J/m2 UV light (•). Then cells were immediately labeled with 0.8 nmol/L [3H]dFdC (1.5 × 10−2 μCi/mL) (A) and 120 nmol/L [3H]F-ara-A (1.5 μCi/mL) (B) in the presence of 1.0 μmol/L BrdUrd and 10 nmol/L FdUrd for 13 hours. DNA isolation and CsCl ultracentrifugation were performed as described in Materials and Methods. Fractions were collected for the measurement of radioactivity.
