Fig. 4.
![Fig. 4. Effects of UV irradiation on [3H]ara-C incorporation into DNA. Exponentially growing CCRF-CEM cells were treated without (○), or with 10 J/m2 UV light (•). Then cells were immediately labeled with 0.6 nmol/L [3H]ara-C (1.5 × 10−2 μCi/mL) in the presence of 1.0 μmol/L BrdUrd and 10 nmol/L FdUrd for 4 hours (A) and 13 hours (B). DNA isolation and CsCl ultracentrifugation were performed as described in Materials and Methods. Fractions were collected for the measurement of radioactivity.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/1/10.1182_blood.v90.1.270/5/bl_0051f4.jpeg?Expires=1722791875&Signature=08Z0Jz11azY0kt2vwFP2MDn510bzfR4DtQMgORvyB~ppIoPcsxeTA6d6GucycUmP9ZCRoesKrqr7rdZQgMKG4qm6siK24d6Wj6iZMX5Ial-WhBFF~QfN6HRbSHB84B9f8WNh8U~DX~PjgPMSjcco5ilvj2qqdUFKF4GdlMwQMaDX2-YizRfqfCayGlf-YlZcFydtuO1ohx69rOgCqVnJYJ0SabmLqWS992anT4ufJQ0zlHN2GkxhyuEVKv8UBF542lf-N99NPBStzTjgwCBO3IlkMHj0YzAZ6oQ8vo1yGCXb-RxiPhtD3-JYsdI-eS1Xbb8PgVQUBHsx8U8obbNDZg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of UV irradiation on [3H]ara-C incorporation into DNA. Exponentially growing CCRF-CEM cells were treated without (○), or with 10 J/m2 UV light (•). Then cells were immediately labeled with 0.6 nmol/L [3H]ara-C (1.5 × 10−2 μCi/mL) in the presence of 1.0 μmol/L BrdUrd and 10 nmol/L FdUrd for 4 hours (A) and 13 hours (B). DNA isolation and CsCl ultracentrifugation were performed as described in Materials and Methods. Fractions were collected for the measurement of radioactivity.
