Fig. 5.
![Fig. 5. CrmA expression protects CEM-C7H2 T cells from being killed by malignant plasma cells. CrmA-transfected T cells (clone 2E8, □) and T cells carrying the relevant vector control (clone IC3, ▪) were labeled with 3[H]-thymidine for 5 hours and cocultivated with the unlabeled neoplastic plasma cell lines for 72 hours using the predetermined E/T ratio of 10:1. Treatment of T cells with 0.25 μg/mL anti-Fas MoAb served as an internal control for the assay. Mean values ± SEM (n = 8) are given. The decrease in DNA-incorporated radioactivity was used to calculate the percentage of myeloma cell–mediated target cell death. Mean values ± SEM (n = 8) are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/1/10.1182_blood.v90.1.12/5/bl_0055f5.jpeg?Expires=1723441697&Signature=47xr8qPa5IS6pKoDueQcExhYR8MZfxqLTHaEwYCnE6Qg7QN0x3HY-91c1jTp~dHB2bfYNYGYoCAdT94a35vjTtWfeKczzG7Gbjee9s11NXtTk4jKMw0QEryfK1V83dKMIyhe3GhxFfIG~TIzhJCjWXRavRbqzijvYhiaLFZ3GgYNKc9NgAyvHImySRmeiO6ycmujVladj4UNEN8imhg6N~YmKlxGdMBVHXC5k35sM23yBuDds8xe8iLFT~OEc8ANHSXAw6Qbk1791HGBICDgZcUUln0Fk0ZUCGxYiz~FmNA79xEbfrN~W4raZ2JJoq1kG3jxTXVEDSGAi-TatEahzg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CrmA expression protects CEM-C7H2 T cells from being killed by malignant plasma cells. CrmA-transfected T cells (clone 2E8, □) and T cells carrying the relevant vector control (clone IC3, ▪) were labeled with 3[H]-thymidine for 5 hours and cocultivated with the unlabeled neoplastic plasma cell lines for 72 hours using the predetermined E/T ratio of 10:1. Treatment of T cells with 0.25 μg/mL anti-Fas MoAb served as an internal control for the assay. Mean values ± SEM (n = 8) are given. The decrease in DNA-incorporated radioactivity was used to calculate the percentage of myeloma cell–mediated target cell death. Mean values ± SEM (n = 8) are shown.
