Expression of lineage-specific genes in Ptpγ transfected ES-D3 derived hematopoietic colonies. RNA was isolated from 20 individual colonies 14 days after plating 7-day EB cells in methylcellulose medium; for each condition gene expression was evaluated by RT-PCR and the percentage of colonies that express the indicated marker are shown. Open bar, pXT1/S, represents either ES-D3 S5 or S9 Ptpγ expressors; striped bar, pXT1, represents ES-D3 transfected with empty vector. The results are the average ±SD of two independent experiments.