Hematopoietic colonies from Ptpγ overexpressing ES clones. Number of colonies derived from the plating of 10⁴ isolated ES-D3 cells tranfected with empty vector (pXT1) or full-length Ptpγ in the sense orientation (pXT1 S5 and S9). Cells from EBs differentiated for 0, 3, 7, 10, and 14 days were plated in semisolid methylcellulose, and the developed colonies were scored 2 weeks later. The results represent the average ±SD of two independent experiments. X-axis indicates days of differentiation before plating ES cells in methylcellulose medium.