Fig. 3.
![Fig. 3. Recombinant Sp1 protein bound the core promoter to form the same DNA-protein complexes as nuclear extracts from K562 cells or from K562 cells induced with phorbol dibutyrate. Gel mobility shift experiments were performed using 32P-end–labeled core promoter (bp −30 to −92) and either recombinant Sp1 protein (at the indicated footprinting units [fu]) or nuclear extracts from uninduced and induced K562 cells. A footprinting unit is defined as the amount of protein required to yield a complete footprint on a fragment of the SV40 early promoter. Two DNA-protein complexes are indicated by arrows.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/2/10.1182_blood.v90.2.678/4/bl_0025f3.jpeg?Expires=1724220774&Signature=AeRhLo1QqPH98xAoXrUaViFy3QrtGcJjeyt2-hZs7rj7g59vWz2hcD035slUoiux9JeAhlrAo98sAU5zadtRbVk-CSV3MIf8aR7ROzrYAiKVVUtch-UxkrVM0ajJ39xk-7PBcBg3qJTpELSU5A3zZxHSQA1BvFpaHiEtkmjucUYNRjqpPz0pZFy5puHEkUBLOpr1aEXU-zFs0gCATIFeu3EVqOoFA73PbkhjYgV41Oyvk-gFZEBPKROx07txWUWqrFA~skmYS48TGCrQH3k88Qgf8TTIhd9Ztk6FGEZrN~E-BrhmGdjtp4XRGJhQD2u~8xraaNNxMrHP5WSc1whzzQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Recombinant Sp1 protein bound the core promoter to form the same DNA-protein complexes as nuclear extracts from K562 cells or from K562 cells induced with phorbol dibutyrate. Gel mobility shift experiments were performed using 32P-end–labeled core promoter (bp −30 to −92) and either recombinant Sp1 protein (at the indicated footprinting units [fu]) or nuclear extracts from uninduced and induced K562 cells. A footprinting unit is defined as the amount of protein required to yield a complete footprint on a fragment of the SV40 early promoter. Two DNA-protein complexes are indicated by arrows.
