Fig. 4.
Dose response of MoAb90-induced B-cell apoptosis. Resting B cells were cultured for 5 days over irradiated CD32-L cells in the presence of 1 μg/mL anti-CD40 MoAb. Increasing concentrations of control IgG1, W6/32, or intact Ig, F(ab′)2 , and F(ab)′ fragments from MoAb90 were then added to the cultures. Cells were collected after 2 hours of treatment with the different MoAbs and the percentage of DNA fragmented cells was measured by TUNEL assay.
