Fig. 3.
![Fig. 3. Scatchard plots of [125I]–GM-CSF binding to target cells. In (A), N87 cells (1.3 × 106/200 μL/well) were incubated with 0.15, 0.3, 0.6, 1.2, 2.4, and 4.8 nmol/L [125I]–GM-CSF (5.7 μCi/μg) in RPMI containing 0.1% bovine serum albumin and 0.2% NaN3 for 30 minutes, washed, and counted. HL60 cells (B) were assayed similarly at 2.2 × 106/well.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/1/10.1182_blood.v90.1.252/5/bl_0029f3.jpeg?Expires=1722791822&Signature=moN9d9zZy3OfAVwiQ4-nlPTByr0Gg9imlFAdLNTFH3GG7lGztRun8iBgdieRibNzo7-2kZu~0x1kumUCVidmO~GAsHy7sQ7~8dqMGpgs4Rit~3hxZSvmbbx77Bj8E7okr-M4TnMApL-1Bot1RCVuvl~IMKnVpgFj6eu8VFbRpPiBwF6SGVUiCb4NkMYLoibv4Sbmio8xIVNLFOBnFHnphh-RRLfgrhiaw-ugCMOsZ6mwjJDOktW4p47EWR6wHe~k3vcrSRUTHAPBXNVlsbeMo~ie3Iw3nQTnf2HqR0KWFf1UA3kDMPsajmaOLusg5Z3rFtMI7tlTRHvdE9EdGn5YFQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Scatchard plots of [125I]–GM-CSF binding to target cells. In (A), N87 cells (1.3 × 106/200 μL/well) were incubated with 0.15, 0.3, 0.6, 1.2, 2.4, and 4.8 nmol/L [125I]–GM-CSF (5.7 μCi/μg) in RPMI containing 0.1% bovine serum albumin and 0.2% NaN3 for 30 minutes, washed, and counted. HL60 cells (B) were assayed similarly at 2.2 × 106/well.
