Western blot analysis of Bcl-2 protein family in FVA cells. The left side of each panel shows the protein levels after 2, 4, 8, and 12 hours of EP-deprived (−) and EP-exposed (+) FVA cells. The right side of each panel shows the long-term developmental expression of proteins in EP-treated cells. These lanes depict cells cultured with EP for 4, 12, 18, 24, 36, and 48 hours. The top set of panels shows blots probed simultaneously using 5 μg each of rabbit polyclonal anti–Bcl-x and anti-Bax antibodies: Bcl-X (29.2 kD) and Bax (21 kD). The second set of panels shows blots probed using 5 μg of mouse monoclonal anti-Bad antibody: Bad (22.1 kD). The bottom set of panels shows blots probed using 5 μg of mouse monoclonal anti–Bcl-2 antibody: Bcl-2 (26 kD). Far right lanes of each blot of the bottom panel contain proteins from HL60 cells that were used as a positive control for Bcl-2 (26 kD).