Fig. 7.
![Fig. 7. In vitro transcription, translation, and transport of ALAS-E precursor into mitochondria. Wild type (lanes 2 and 5) and V190 mutant ALAS-E (lanes 3 and 6) precursors were synthesized in the presence of [35S]-labeled methionine. Lanes 1 and 4 are controls in which the RNA was omitted. The translation products were incubated with mouse liver mitochondria and then centrifuged to collect the mitochondria. Supernatant (lanes 1 to 3) and mitochondrial (lanes 4 to 6) fractions were analyzed by SDS-PAGE. Note that the sizes of the precursors are similar, but the processed mutant proteins are larger than the wild type.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/2/10.1182_blood.v90.2.822/4/bl_0035f7.jpeg?Expires=1723368052&Signature=0p~Nq9CBEELbYXCi5NM3I-sH-mAIyX2mzHUHHY21ngrv9G0drvGgV9i0lvryMYhyZIGxJFCfUvDGio1d3pj-aBYoSbpnfB5-d7GizPpDmDfSM5SkdLHjAdjgmjhoZokhqbb94T5lEu1IrQBRnsqubfLVYiZ5lGMTDaRb6XBPEBJUnGfo8MPkSvcX7fu7uwCGuu-VBBKecuY4RHUelJ295GTK0LPQDOhIPHgrpJER4TlAcAyFsenwX0dCVqOITl4L793vGR~MFyz8KSAjZTPXWS7AtDYmIkhlTKRD7EqYvJL0xlsFTSh2FtgeNvcsKX~dFtgK0uH71wg8vvstt2YueA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
In vitro transcription, translation, and transport of ALAS-E precursor into mitochondria. Wild type (lanes 2 and 5) and V190 mutant ALAS-E (lanes 3 and 6) precursors were synthesized in the presence of [35S]-labeled methionine. Lanes 1 and 4 are controls in which the RNA was omitted. The translation products were incubated with mouse liver mitochondria and then centrifuged to collect the mitochondria. Supernatant (lanes 1 to 3) and mitochondrial (lanes 4 to 6) fractions were analyzed by SDS-PAGE. Note that the sizes of the precursors are similar, but the processed mutant proteins are larger than the wild type.
