Schematic representation of RHD Intron 4/exon 10 multiplex PCR. A diagrammatic representation of the multiplex assay used in this study is depicted in this figure. The approximate positions of the amplimers are shown with the identification letters (A through D) shown at the 5′ end of the primer. The sequences of the primers are shown and their nucleotide positions (numbered within either RHCE or RHD intron 4 (Fig 2); or corresponding to Rh cE4,5 or D⁶ cDNAs). The location of the RHD specific deletion or RHCE specific insertion are highlighted on the figure.