Flow cytometric determination of TM. HUVECs treated with staurosporine (left panels) or kept in suspension with serum deprivation (right panels) for 2, 4, and 8 hours were harvested, washed, and stained with an antihuman TM MoAb, followed by an FITC-conjugated goat antimouse antibody. The M1-region is defined as a percentage of events below the control (all panels, right peak). The results of one representative experiment of three performed are shown.