Ristocetin- and botrocetin-induced platelet agglutination. (A) PRP from the patient failed to agglutinate with ristocetin at 1.2 mg/mL and botrocetin at 5 μg/mL. The agglutination response of the mother was slightly impaired compared with that of the normal controls (n = 5). (B) Washed platelets were suspended in normal platelet-poor plasma and the platelet agglutination response was also evaluated. Platelets from the patient agglutinated to ristocetin at 1.2 mg/mL and botrocetin at 5 μg/mL. Values for the normal controls are the means ± SD. (○) Normal controls; (□) father; (▵) mother; (•) patient A.K.