Restriction enzyme analysis of G-CSF receptor RT-PCR products from neutrophils of patient MA and her family members. Neutrophils from congenital neutropenia patient MA who developed secondary AML (lanes 1 and 2), her brother FA, also a congenital neutropenia patient, (lanes 3 and 4), their healthy mother (lanes 5 and 6), and father (lanes 7 and 8) were used for RNA isolation and subsequent RT-PCR analysis. PCR fragments amplified with primers Q1 and P2 are either shown undigested (lanes 1, 3, 5, and 7) or digested with PvuII (lanes 2, 4, 6, and 8) at 37°C overnight and after agarose gel electrophoresis and ethidium bromide staining. Numbers on the right indicate the undigested (601) and the digested (475) PCR fragments. The smaller digested fragment of 126-bp is not shown. DNA fragments lengths standard M.VI from Boehringer Mannheim was loaded in lanes M (2176, 1766, 1230, 1033, 653, 517, 453, 394, 298, 234 bp).